Milk allergens were digested by allowing them to flow through a chromatography column, where pepsin was conjugated to the stationary phase of the column. The allergen fragments were then characterized both biochemically, by using SDS-PAGE and gel permeation chromatography, and immunologically, by examining their reactivity to IgE and monoclonal antibodies..

Poly- and perfluoroalkyl substances (PFASs) are a group of environmental contaminants that have gained increasing attention due to their potential to bioaccumulate, environmental persistence and potential toxicity. PFASs have been found in surface water, sediment, air, soil, sludge and ice caps globally, as well as in wildlife and humans. Furthermore, PFASs have also been detected in drinking water, leading to raised concerns for human health, since drinking water is one of the most significant sources of PFASs for the general population. Conventional water treatment techniques have shown to be ineffective removing PFASs, highlighting the importance for further research to develop efficient removal techniques. The aim of this study was to investigate the removal efficiency of PFASs in water using two treatment techniques; granular activated carbon (GAC), type Filtrasorb 400®, and anion exchange (AE), type Purolite A-600. Additionally, the effect of dissolved organic carbon (DOC) on removal efficiency was studied.

Since the beginning of 1940 there have been externally developed tests in Sweden. Their appearances have changed over the years together with the cognitive approach. Nowadays, this kind of tests is going by the name of the national tests, which was introduced in the year of 1994. When the Swedish National Agency for Education received the assignment to develop new purposes and national tests to the third grade students, debate took a bounce. The matter was well discussed; national tests in third grade or not?The national tests thus far carried out four rounds in the third grade and the purpose of my study was to find out how teachers are reasoning about the tests.

The purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macrocyclic glycopeptides (Chirobiotic V, V2 and T) and polysaccharides (Lux Cellulose-1, Cellulose-2 and Amylose-2).The Chirobiotic V column showed high selectivity for citalopram and its metabolites. High resolution was obtained using a mobile phase consisting of methanol, acetic acid and ammonia. High selectivity for the enantiomers of zopiclone and its metabolites were obtained on the Cellulose-2 column using a mobile phase consisting of acetonitrile and ammonium acetate buffer.The enantiomers of tramadol were separated with the Amylose-2 column. However, changes in the pressure arose, probably caused by the additive NH4HCO3.

National tests has been carried out in fifth grade since 1996. Spring 2011 is the first year since the start, when it is no longer mandatory for Swedish schools to carry out the tests in fifth grade. Instead the tests will be moved to sixth grade, starting in spring 2012. This thesis investigates what effects the national tests has got on the teachers planning, but also what attitude the educators have to the tests. To solve the subject, teachers that are active in fifth grade and have recently carried out nationel tests in their class, were interviewed.

Different field tests are currently used in a wide extent in different levels of soccer teams. To be able to assess the players? physiological status the tests must have high validity, reliability and external validity. Many different tests have been developt, but currently only five of these tests are most commonly used: Yo-Yo intermittent recovery test (YIRT), Yo-Yo intermittent endurance test (YIET), Multi-sprint-test, Beep-test and Cooper-test. Presently there is no definitive conclusion regarding which of these tests is most suitable for soccer players.

In the year 2010 a transition will take place here in Sweden from the present rules how to dimension buildings in to the common rules with have been developed in Europe, the Eurocodes. Eurocode is the term for a collection standard that contains calculation rules in how to dimension constructions and buildings. They are developed by the European standardize committee.The purpose with this examination work is to get an insight of what changes this will contribute to, and how it will affect the dimensioning. Are there going to be any differences in the dimension you finally chose?To investigate these possible differences two constructions will be calculated first in the present Swedish rules, and then in the coming eurocodes.One of these two constructions will be build completely in steel, while the other will be build completely in concrete.

In this project a LC-MS-method for the analysis of gamma-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, amphetamine and methadone was developed.Initially, the efficiency of the ionisation of the analytes was evaluated with respect to the ionisation technique (ESI, APCI and APPI) and the composition of the mobile phase. In the next step a number of different columns was tested in order to find the one with the greatest potential for separation of the substances in question. Using the selected column, the separation was optimised by means of experimental design and the software The Unscrambler 7.8. The parameters studied were the flow rate, the column temperature and the mobile phase composition. The response variables were the resolution between the target compounds and the retention time of the last eluting compound.These experiments showed that, in order to obtain the best ionisation, the mobile phase should consist of 5 mM formic acid in water and acetonitrile.

Förorenat dagvatten från Stena Gotthards metallåtervinningsanläggning i Eskilstuna har filtrerats genom kolonner innehållande två olika adsorbenter, torv och furubarksflis. Torv är en organisk jordart som främst används som bränsle och furubarksflis är en biprodukt från skogsindustrin. Båda filtermaterialen är relativt billiga och finns i stora kvantiteter i Sverige. Filtermaterialen har visat sig ha varierande kapacitet att adsorbera metaller i vatten på grund av själva materialet samt vattnets sammansättning. Resultatet från den här studien visar att av de två undersökta filtermaterialen så har furubarksflis störst kapacitet att reducera bly, koppar och zink från det specifika dag vattnet..

During the past few years there has been an observed decrease in physical fitness within different patient groups. As a part of the rehabilitating physiotherapy treatment it is important to acknowledge the physical condition of the patient. Physical fitness testing might be difficult due to physical limitations within different patient groups. A broad range of exercise tests is therefore of importance in clinical practice. The aim of this overview was to describe exercise tests used in rehabilitation contexts, and to define areas of use and restrictions.

An excessive input of nutrients to lakes and other water bodies has created a problem with eutrophication in Sweden. Untreated, or partially treated, domestic sewage is a major source for discharge of phosphorus (P), which is the nutrient most frequently responsible for eutrophication of most fresh waters and the Baltic Sea. The waste water can be cleaned by filter materials, which have a high P-retention ability and which after saturation may be used as fertilizers. Four potentially suitable filter materials were tested in batch- and column experiments in this study. In the batch experiments, the following materials were shaken with waste water in time series ranging from 5 seconds to 60 minutes: coarse (1-4 mm) and fine (0-2 mm) Polonite® (heated bedrock from Poland); Filtralite® (light expanded clay aggregates with limestone added before burning); water cooled blast furnace slag (BF-slag) and BF-slag mixed with 10% burned limestone.

This essay aims to study how national tests in the ninth grade are being viewed upon in the Swedish school system. What are the affected people?s thoughts regarding its field of application? For further information about this matter, three different sub studies have taken place. In the first sub study, literature from The Swedish National Agency for Education (SNAE) has been studied and compared to other researches. In the second sub study, pupils who took part in the national tests during the previous year answered a survey about their experiences working with the tests.

A significant amount of a teacher?s work consists of evaluation and marking of the students, which involves a great responsibility. This essay put the teacher?s evaluation of the students in focus. Today?s course Idrott och hälsa A, at the upper secondary school level, consists of 100 points and all students take this course apart from students attending apprentice programs.

This thesis describes how the chip space and coolant channels are designed in today?s mills, the ideas behind the design and what is considered to be the best method of cooling. It contains ideas on how the chip space can be simplified. It contains several concepts on how the cooling could be designed for better efficiency and manufacturability. There are also suggestions on how the cooling could be stopped when the cutter isn?t cutting.

Four different kinds of filter-materials with reactive surfaces have been studied concerning their capacity to absorb heavy metals in leachate from a municipal waste deposit. The heavy metals studied were: lead, cadmium, copper, mercury, chromium, nickel and zinc. The leachate contains high levels of dissolved organic carbon (DOC) and has a high pH-value along with a high buffer capacity. These characteristics of the leachate make it difficult to remove pollutants and require efficient filters. The filters that were examined in the report are blast-furnace slag with CaO, sand covered with iron oxides, olivine and nepheline.